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(i) Adult neurogenesis has been assessed by different cell markers indicating proliferation (Ki-67), juvenile stages of neurons (DCX), and slowly dividing precursor cells (MCM2).
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Interestingly, authors compared their single-cell transcriptomes with those from similar cells [NSCs and transit amplifying progenitors (TAPs)] sorted with different cell markers [22].
They are defined by different cell surface markers and characterized by specific phenotypic traits.
The characteristics of tumors induced in NG2-KO were compared to those of tumors induced in wild type mice by immunostaining for different cell lineage markers and by transplantation assays in adult mice.
This procedure was considered necessary to be able to compare density of positive cells over time, between different locations and different immune cell markers.
B-cell subpopulations are characterised mainly by the differential expression of different cell surface markers that include various cluster of differentiation (CD) molecules and different surface immunoglobulin isotypes (B-cell antigen receptor).
Uterine tissue and endometriotic lesions were isolated and dissociated into single cell suspensions, and endothelial cells were identified using flow cytometry by the expression of two different cell surface markers (CD31+ and VEGFR2+) and exclusion of a hematopoietic cell surface antigen (CD45−).
It has been proposed that cell-cell recognition occurs by means of local cell surface modulation of a small number of proteins rather than by expression of large numbers of different cell surface markers.
Among the 13 different NK cell markers studied by flow cytometry analysis, the expression of CD244 and NKG2D increased significantly in the CD56bright NK population.
Nonparametric Spearman's rank correlation coefficient test was performed to determine the association between MV identified by two different cell surface-specific markers and to determine age-associated changes in blood-borne MV numbers.
Similar finding was also demonstrated by the other group that breast cancers may contain tumor initiating cells displaying different cell surface markers (Wright et al., 2008).
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