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The results showed that the DNA extraction procedure was not affected by different cell concentrations within the tested range.
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Figure 1 Effects of different cell concentrations on bromelain activity by BL21-AI.
To achieve a suitable occupancy frequency of one cell per well, we identified optimal cell concentration by plating different cell densities into Terasaki plates using a principle similar to limiting dilution cloning [ 18].
For example, Niu et al. [ 38] introduced a caspase-3 specific cyclic firefly luciferase reporter gene (pcFluc-DEVD) into UM-SCC-22B and 4T1 cells then induced apoptosis of the cells by different concentrations of doxorubicin and evaluated the effect by BLI.
First, we tested whether the migration of metastatic NSCLC cells was affected by different collagen concentrations.
After the incubation, control cells as well as MC3T3-E1 cells treated by different types and concentrations of β-SiC nanowires were harvested, gently washed with 0.01 M PBS (pH 7.4), and fixed by 2.5% glutaraldehyde for two hours.
We measured downstream Gli1 expression induced by different concentrations of SAG in C3H10T1/2 cells in the presence of different concentrations of the inhibitors 1 (robotnikinin), 25 and 29, as well as the Smo antagonists cyclopamine and GDC-0449 (vismodegib).
To compensate the enzyme concentrations from different cell growth, the 3-HP production rate (ΔA/min) was divided by OD600 for analyzing the results.
Surprisingly, the majority of differentially expressed genes/transcripts modulated by different concentrations of I-IUdR in cell culture media being induced or repressed by either 3.7 kBq/ml or 18.5 kBq/ml concentrations of I-IUdR are distinct.
The function of β-adrenergic receptors was demonstrated in the cell membranes of breast cancer cells by the significant increase in cyclic adenosine monophosphate (cAMP) production induced by different concentrations of isoproterenol compared with cells that were unstimulated (control).
While MVA-NAO enabled comparing cell death induced by different concentrations of a single treatment, in most screens the effect of unknown compounds is compared to a single positive control.
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