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Importantly, phlebotomy was performed in a clinical research unit precisely 12 hours after IFN injection, minimizing variability induced by differences in sampling times.
These variations can be partly explained by differences in sampling strategies.
It was suggested that the differences in the prevalence of AS might partly be explained by differences in sampling with regard to the surveillance streams and differences in the age structure or PrP genotype distribution of the population.
The sampling bias might still account for the high heterogeneity of the African strains, but the differences in diversity for CA and ME strains cannot be explained only by differences in sampling.
Comparisons of zooplankton diversity and communities over time and space have been hindered by differences in sampling designs and collection methods, as well as by limitations in sample analysis and access to data [21][22].
Whereas the Victoria lineage experienced strong seasonal fluctuations in relative genetic diversity, little change was observed over the same time period for the Yamagata lineage, and these observations were not heavily affected by differences in sampling density.
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Since estimates of genetic diversity D are not affected by differences in sample size, direct comparisons between different studies are possible.
This sub-sampling step was conducted to avoid a bias introduced by differences in sample size between the two data sets.
This difference may be partly explained by differences in sample variety in their study group.
Allelic richness was calculated using CONTRIB [62] with rarefaction to the smallest sample size obtained minus 1 to produce estimates that are unbiased by differences in sample sizes.
This discrepancy might be explained by differences in sample processing.
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