Sentence examples for by dideoxy sequencing for from inspiring English sources

Tumour DNA was then analysed by dideoxy sequencing for mutations in K-Ras exons 1, 2 and 3 (including the hotspot codons 12 and 13 (exon 1), 61 (exon 2) and codon 146 (exon 3)), PIK3CA (exons 9 and 20) and B-Raf (V600E).

The sequence of the cloned gene was confirmed by dideoxy sequencing of both strands.

The sequence ambiguities and gaps were resolved by dideoxy sequencing of PCR amplicons using total cellular DNA as template, and the genome sequences were deposited in GenBank.

DNA sequence of trnQ- cemA region in 10 M. truncatula lines (GenBank KC989947 KC989956) was determined by dideoxy sequencing of PCR amplicons.

The authenticity of all constructs was verified by dideoxy sequencing using ALF sequencer (Amersham Pharmacia Biotech, Piscataway, NJ).

PCR products were cloned into the pCR®4-TOPO Vector (Invitrogen) and the DNA sequence of the 5'-prime-end was determined by dideoxy sequencing between 21 and 42 clones for each transgenic line.

Although 'brute force' DNA genotyping by dideoxy sequencing has been the method of choice for de novo SNP detection, it suffers from difficulty of DNA template amplification, limitations of PCR multiplexing per reaction, and increasing equipment costs.

The results obtained for tumor samples were 95% comparable to those obtained by dideoxy sequencing.

All promoter sequences generated were verified by dideoxy sequencing.

Pyrosequencing confirmed all mutations detected by dideoxy sequencing (Table 1).

Positive mutant clones were confirmed by dideoxy sequencing.