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We solved this problem by developing a sorted nuclei ChIP-seq pipeline.
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Q-methodology involves 3 stages: developing a set of statements to be sorted; sorting of those statements by participants along a continuum of agreement; and analysis and interpretation of the sorted data [ 28, 29].
I've got this one sorted, lads, like the joke about the Russian scientists who sent a cosmonaut to the moon with a pencil while Nasa faffed around developing a super-sophisticated space-compliant pen.
We developed a customized pipeline that takes individually sorted raw sequences and outputs files ready for SNP calling.
Embryos were sorted based on their phenotype; mutants develop a swollen pericardium and lack fin buds.
We have developed a preliminary knowledge base for qualitative reasoning about containers, expressed in a sorted first-order language of time, geometry, objects, histories, and actions.
In order to construct BAC libraries from DNA of sorted plant chromosomes, Šafář et al. (2004) developed a protocol which requires only a few micrograms of DNA.
We have developed a method of studying the organization of the genome by analysis of the 3D organization of telomeres in the nucleus and their positional changes along the cell cycle, using flow-sorted living cells.
Our novel contributions are: (i) we develop a new pattern-selection based method to detect the regions of CNA for a heterogeneity disease such as MDS by using sorted bone marrow SNP arrays.
It is separated from the basal unit of well-sorted and well-rounded coral rubble (lower left close-up photo) by a palaeosol developed in a layer of poorly sorted carbonate sand and coral material (close-up photo on the right).
To conclude, the pipeline we have described here was developed with the aim of producing a sorted, enriched subset of true direct regulatory interactions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com