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The leakage of cytoplasmic membrane was analyzed by determining the release of cell materials including nucleic acid, metabolites and ions which was absorbed at 260 nm in the suspensions (Oonmetta-aree et al. 2006).
The encapsulation of calcein was confirmed in the fluorimeter by determining the release of the self-quenched calcein upon the addition of 0.5% (v/ v) Triton X-100.
Degranulation was monitored by determining the release of β-Hex because histamine release by activated mast cells parallels the release of β-Hex.
Alkaline phosphatase (ALP) activity was assayed in cell layers by determining the release of p-nitrophenol from p-nitrophenylphosphate (Sigma) at 37 °C and a pH of 10.5.
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In the investigated parameter field, several regimes with different types of reaction can be identified by determining the heat release rate inside the reactor.
The polydispersity index (PDI) was 0.25 and 0.19 for FA and ASP c-SLN, respectively (Table 1).> -wrap-foot> c-SLN chitosolidolipidipid nanoparticle The ability of nanoparticles to deliver drugs was examined by determining the drug release in PBS solution (pH 6.8) and acidic conditions (pH 1.6), as shown in Fig. 1.
Proteolysis was measured by determining the rate of release of trichloroacetic acid (TCA -soluble proTCA -solublectively labeled with [3H]-tyrosine into the media.
The hemolytic activity of NZ2114 was evaluated by determining the amount of released hemoglobin from a 4% suspension of fresh mice red blood cells (RBCs) (Cho and Lee 2011).
The fracture toughness was quantified by determining the critical strain energy release rate (GIC) using the modified beam theory.
Tsetse fly saliva exerts ATP(D ase activity as illustrated in kinetic assays using ATP and ADP as substrates followed by determining the inorganic phosphate (Pi) release.
The effects of each separation step were evaluated by determining the glucose and xylose released (mg g−1 fraction).
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