The total number of organisms in a biofilm was calculated by determining the ratio of killed cells to the total cells with the mean value on 3 images and standard deviation.
Relative resistance factors were calculated for the sub-populations by determining the DMF required for 90% kill compared with that of the B sub-population.
The mechanism of bortezomib-induced clonogenic cell kill was investigated by determining the protection afforded by treatment with antioxidants.
The CD50 (cytotoxic dose that kills 50% of the cells) was calculated by determining the inverse dilution of the bacterial sample that was required to kill 50% of the Vero cells.
In vitro kill curves were constructed by determining the number of viable cells at each time point and MOI by trypan blue exclusion.
Next, we determined the cell kill efficiency of rapamycin and Nimotuzumab in comparison to TMZ in the EGFR-null Gli36 cells, as confirmed by western blot analysis.
On the basis of this approach, the interaction between the three drugs was quantified by determining a combination index (CI) at increasing levels of cell kill.
Albinos text in their location if they suspect they are being tracked by gangsters determined to kill them and harvest their body parts.According to the Tanzania Albino Society, at least 35 albinos were murdered in Tanzania last year to supply witch doctors with limbs, organs and hair for their potions.
They used the terrorist assault on Mumbai in November 2008 as their model, an invasion of a crowded public building by gunmen determined to kill as many people as possible in a short time.
The time-kill assay, as described by Sopirala et al. (2010), with some modifications, was used to determine the time that B. pseudomallei was killed after N2-4 and N3-8 precipitated proteins were added into the B. pseudomallei cultures.
