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The cytotoxicity of the treatments was measured by determining the activity of lactate dehydrogenase (LDH) enzyme released into medium with the CytoTox96® Non-Radioactive Cytotoxicity Assay (Promega, Fitchburg, WI, USA).
Then, the ANN procedure was checked by determining the activity levels of different spectra obtained from certified samples for which one knows a priori the radioactive content, and its results were compared with those of other methods.
In this study, toxicity assessment tests have been carried out by determining the activity of dehydrogenase enzyme reducing Resazurin (RR) and colony forming unit (CFU) methods.
Cell membrane integrity of MDA-MB-231 cells was evaluated by determining the activity of lactate dehydrogenase (LDH) leaking out of the cell, according to the manufacturer's instructions (in vitro toxicology assay kit, TOX7, Sigma, USA).
Cell membrane integrity of PMEF cells was evaluated by determining the activity of lactate dehydrogenase (LDH) leaking out of the cell according to manufacturer's instructions (in vitro toxicology assay kit, TOX7, Sigma-Aldrich).
These contrasting results were further investigated by determining the activity periods of Mariner2_Ml in each taxon.
Similar(47)
The temperature profiles for the activities of xylanases were obtained by determining the activities at different reaction temperatures (30, 35, 40, 45, 50, 55, 60, 65, and 70 °C) in 0.1 mol/L McIlvaine buffer (pH 5.0).
By determining the activity-state profile for key enzymes of tau phosphorylation we could identify kinases potentially involved in the differentially regulated, reversible tau phosphorylation that occurs during hibernation.
The effects of EGCG were assessed quantitatively by determining the activities of serum muscle-derived creatine kinase, isometric contractions of triceps surae muscles, integrated spontaneous locomotor activities, and oxidative stress and fibrosis in selected muscles.
The process was assessed by determining the activities of ligninolytic enzymes produced by qualified organisms at different time periods and through their association with concomitant OMW decolorization, reduction of phenolics, and decrease of plant toxicity.
Antioxidant status in both the liver and kidney tissues were estimated by determining the activities of the antioxidative enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST); as well as by determining the levels of thiobarbutaric acid reactive substances (TBARS) and reduced glutathione (GSH).
More suggestions(18)
by determining the intra
by determining the level
by determining the correlation
by determining the slope
by determining the percentage
by determining the number
by determining the ratio
by examining the activity
by determining the diameter
by determining the currency
by determining the phylogeny
by determining the sector
by regulating the activity
by determining the difference
by determining the product
by determining the mass
by determining the oxidation
by determining the threshold
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CEO of Professional Science Editing for Scientists @ prosciediting.com