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I-Sce I activity led to effective intracellular processing of viral DNA as confirmed by detection of specific cleavage products.
Therefore, in order to determine whether the yeast isolates show pathogenicity, it was studied by the detection of enzymes like protease, phospholipase, the ability of haemolysis and by detection of specific pathogenic genes through polymerase chain reaction (PCR) amplification.
Acute infection was confirmed by detection of specific IgM antibodies in 47 patients.
This was followed by detection of specific staining with the Dako LSAB2 HRP system with DAB (diaminobenzidine) substrate.
The diagnosis of tularemia was confirmed by detection of specific antibodies by microagglutination test and/or F. tularensis nucleic acid in a clinical specimen (lymph node aspirate).
We found that TSCs were able to differentiate into specific lineages of cells (adipocytes, chondrocytes, and osteocytes), which was verified by detection of specific marker gene expression.
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We use the digitalized RiO to conduct a phenotypic screen on a pool of RiO, followed by detection of each specific donor in situ.
The presence of the virus and its multiplication in Vero cells was confirmed by detection of viral specific DNA and antigen by using polymerase chain reaction (PCR) and indirect immuno fluorescent assay (IIFA), respectively.
However, microarray analyses of gene expression, although commonly used, often yield divergent data and, therefore, require direct confirmation by detection of the specific transcripts.
The power of a more precise bin approach is illustrated by detection of a specific isoform of Adora 3 consisting of only two exons out of a total of 9 known for this locus and upregulated at the 3 hour time point (Additional file 6: Figure S3).
These immune responses can be evaluated by detection of TAA-specific immunoglobulin or TAA-specific T-cell responses such as γ-interferon (IFN-γ), tumor necrosis factor-α (TNF-α), and interleukin-2 (IL-2) secretion by T cells.
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