Sentence examples for by detecting the fluorescence from inspiring English sources
ROS were measured by detecting the fluorescence intensity of carboxy-H2DCFDA (5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein 5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein 5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein 5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein 5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein 5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein
After purification using glutathione sepharose beads, the 82 kDa fusion protein was separated on the SDS-PAGE gel and confirmed by detecting the fluorescence through luminescent image analyzer.
Compounds D7 and D10, seco-A-oleanane-3,4-lactone, suppressed the HBeAg secretion with IC50 values of 0.14 μM and 0.86 μM respectively, and the inhibitory activities were also confirmed by detecting the fluorescence intensity of FITC-labeled monoclonal mouse HBeAg antibody via flow cytometry.
Also, it is much easier to assess the integrity of proteins by detecting the fluorescence in SDS polyacrylamide gels.
The ROS level was examined by detecting the fluorescence intensity using fluorescence microscope and microplate reader with excitation an emission wavelengths set at 500 and 529 nm, respectively.
Thus, the level of intracellular ROS can be monitored by detecting the fluorescence of DCF. Figure 6 shows that the fluorescence of lipopeptides-treated cells was much stronger than that of the untreated cells.
Intracellular doxorubicin fluorescence was measured by detecting the natural fluorescence of doxorubicin with flow cytometry (FL2 channel); 10,000 events were scored.
After washing with PBS for twice, a FACScan Flow Cytometer (Becton Dickinson, San Jose, CA, USA) was used to assess the cellular uptake of ADR or ADR-loaded liposomes by detecting the mean fluorescence intensity (MFI) of FL-2 (ADR fluorescence).
Given that hydrogen peroxide was generated along with cholesterol oxidation catalyzed by COD-B, ROS levels were evaluated by detecting the DCF fluorescence intensity.
The detector operates by detecting the diffusely emitted fluorescence in a glass capillary, which is a measure for the concentration of the analyte to be detected.
The redox state of the sample can be monitored by detecting the increase in fluorescence.
