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In one of the processes, CFA is separated into narrow particle size fractions, followed by density separation using a fluidized bed of air, preferable in the absence of water by dry screening, and then an optional further particle size separation.
PBMC were isolated from blood of normal adult donors by density separation using Ficoll-Hypaque [ 24].
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Monocyte-depleted PBMCs were prepared by Ficoll-Hypaque density separation using RosetteSep™ monocyte (CD36) depletion cocktail (StemCell Technologies).
Using sterile technique, neutrophils were isolated from whole blood by density gradient separation using Polymorphprep (13.8% sodium diatrizoate and 8.0% polysaccharide, Axis-Shield, Rodelokka, Oslo, Norway), with centrifugation at 500 g for 40 minutes.
Cells were isolated by density gradient separation using LSM 1077 Lymphocyte separation medium and sorted for CD3+ cellsCD14− CD45RO+ cells using FACS.
Cell suspensions were subsequently passed through a 37 μm nylon mesh followed by density gradient separation using HISTOPAQUE 1083 (Sigma-Aldrich), according to the manufacturer's instructions.
PBMCs were obtained from 13 mL of heparinized whole blood of four adult female New Zealand White rabbits (numbered 1, 2, 4, and 5) by density gradient separation using ficoll-histopaque.
Tumor cells were enriched by density-gradient separation using Percoll (blood) or Ficoll Hypaque (BM) solution.
Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats obtained from healthy donors by density-gradient separation using Lymphoprep™ reagent (Axis-Shield, Dundee, UK).
Peripheral blood mononuclear cells (PBMC) were isolated by ficoll-hypaque density gradient separation, using standard protocols.
Isolation of granulocytes from other haematological cells is usually based on density, with separation using a discontinuous plasma/Percoll™ density gradient being a widely used method [26], [37].
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