Sentence examples for by density centrifugation using from inspiring English sources

Mononuclear cells (MNCs) were obtained from the blood by density centrifugation using Leucosep® tubes (50 ml, with filter, Greiner) and the density gradient media Histopaque® 1077 (Sigma) separation solution.

Following dextran sedimentation, Peripheral Blood Neutrophils (PMNs) were prepared by density centrifugation using Optiprep™ (Axis-Shield, Upton Huntingdon, UK) [30].

Monocytes for DC generation were purified from freshly isolated PBMCs by density centrifugation using Percoll (GE Healthcarespec. grav. 1.130±0.005 g/ml, <25 mOsm/kg H20).

Peripheral blood from healthy volunteers and patients was collected in EDTA Vacutainers, and PBMC were separated by density centrifugation using Lymphosep, lymphocyte separation media (Bio West, Nuaille, France).

In vitro experiments were performed using peripheral blood mononuclear cells (PBMC) isolated by density centrifugation using peripheral blood lymphocyte separation medium (Cambrex, Gaithersburg, MD).

PBMCs were separated by density centrifugation using Biocoll (Biochrom AG).

Peripheral blood mononuclear cells were obtained by gradient density centrifugation using Ficoll-Hypaque [ 10].

Peripheral blood mononuclear cells (PBMC) were extracted by gradient density centrifugation using Lymphoprep at 1600 × g (Axis-Shield, Kimbolton, UK).

Human PBMCs were isolated by gradient density centrifugation using Histopaque-1077 cell separation medium (Sigma-Aldrich) as previously described [ 20].

For cell stiffness measurements, polymorphonuclear cells and peripheral blood mononuclear cells (PBMCs) were separately isolated from whole blood by gradient density centrifugation using Histopaque-1077 solution (Sigma-Aldrich, St. Louis, MO, USt . Louis

Mononuclear cells were isolated by density gradient centrifugation using Lymphoprep (density 1.077 g ml−1; Nycomed Pharma, Oslo, Norway), and centrifuged at 480  g for 15 min at room temperature.