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The expression of TPO in normal and lesion areas of thyroid tissue from both patients were quantitated by densitometry scanning using Image-J software (http://rsbweb.nih.gov/ij/).
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The level of LDH-A was analyzed by densitometry scanning of appeared band on gel using Alpha image analyzer software.
Proteins were visualized using the enhanced chemiluminescence (ECL) method and quantified by densitometry scanning with Quantity One software.
Blots were scanned (PowerLook III, Umax Systems GmbH, Willich, Germany) and signal quantification was performed by densitometry using scanning analysis software (TotalLab TL120, Nonlinear Dynamics, Newcastle upon Tyne, UK).
In some experiments, gels were analyzed by scanning densitometry using UN-SCAN-IT gel (Silk Scientific, Orem, UT) as described previously (30, 31).
Protein was detected by chemi-luminescent signalling (Pierce ECL) and the immunoblots were scanned and analysed by densitometry using UN-SCAN-IT software (Silk Scientific , Inc.
X-ray films were quantified by scanning densitometry using ImageJ software (National Institutes of Health).
The intensity of the bands was quantified by scanning densitometry using software Quantity One-4.5.0.
Relative intensities of the protein bands were quantified by scanning densitometry using ImageJ.
Autoradiography bands were quantified by scanning densitometry using Quantity One Quantitation Software™ (Bio-Rad LAlcobendass, S.A., Alcobendas, Madrid).
The bound antibodies were visualized by chemiluminescence detection and protein levels were quantified by scanning densitometry using TotalLab© software.
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