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Signal intensity was quantified by densitometry analysis of SCN region normalised to background staining.
The morphologic findings were further corroborated by immunoblot analysis of kidney homogenates for collagen I and fibronectin, followed by densitometry analysis of immunoblots (Fig. 3c e), which demonstrated a greater beneficial efficacy of the early than the late Tanshinone IIA treatment.
Release efficiency was calculated (at 48 h post-transfection) as the ratio of supernatant Gag to total Gag, both determined by densitometry analysis of Western blot images using Fiji software.
There were two-fold more HA1 and HA2 proteins in virions of rNDV-HAF, compared to rNDV-HA, as determined by densitometry analysis of the bands.
Increases in PDCD4 phosphorylation levels was variable between patients and ranged from 1.7-fold to 7.4-fold and averaged to approximately 3.4-fold (n = 10), as quantified by densitometry analysis of western blots (Figure 5C).
Exon skipping efficiency was estimated by densitometry analysis of the gel images comparing the density of amplicons from dystrophin mRNA with exon 57 skipping to the native dystrophin mRNA.
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Expression of p75c-Myb was more rapidly downmodulated of p89c-Mybex9b; by densitometry analysis, the half-life of p89c-Mybex9b is ∼90 min, nearly 30 min longer than that of p75c-Myb.
Forty two nanograms of hPRR, by densitometry analysis, were obtained from 1 μg of purified rBmNPV-hPRR at 5 days p.i.. Quantity of rBmNPV-hPRR per mg of protein decreased at 5 days compared to 4 days because of an increase of protein concentration in purified rBmNPV-hPRR at 5 days.
By densitometry analysis, the half-life of p89c-Mybex9b IM-treatedted cells was ∼75 min longer than that of p75c-Myb (110 versus 35 min, Figure 3d).
Levels of PCR products were measured by the densitometry analysis of the gel image using the Bio 1D program.
The levels of PCR products were measured by the densitometry analysis of the gel image system using the Bio 1D program.
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