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Protein expression levels on western blots were quantified by densitometry analyses using the ImageJ software.
The relative band intensities were measured by densitometry analyses using Gene Tool.
The relative band intensities were measured by densitometry analyses using Gene Tool (Syngene Inc., USA).
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Western blot bands were subjected to densitometry analyses using Image J (National Institutes of Health, Bethesda, MD, USA).
Relative expression levels and phosphospecific antibody reactivity were measured by densitometry analyses of blots using Image J.
Protein bands were quantitated by densitometry, and analysed using GeneTools software.
Net integrated pixel density for each spot (an average of duplicate spots after subtraction of average background density) was determined by densitometry and analysed using Quantity One software (ISBE, Sheffield, UK).
Western blot analyses (Fig. 4c) and the ratios of PD-L1 in tumors to PD-L1 in counterpart normal controls (determined by densitometry analyses of immunoblot bands) (Fig. 4d) showed that smokers had higher PD-L1 than nonsmokers.
Band intensities of HIF-1 α were quantified by scanning densitometry and analysed using ImageJ software.
Blots were analysed by densitometry measurements using NIH ImageJ software (http://rsb.info.nih.gov/ij/).
Data were analysed by densitometry using AlphaEaseFC 4.0 and graphs were plotted using GraphPad Prism.
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