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Protein bands were quantified by densitometric scanning using a GS-800 Calibrated laser densitometer (Bio-Rad).
Relative intensities of the labeled bands were analyzed by densitometric scanning using MCID Imaging Research Incc) and CREB-activation was expressed as the ratio between pCREB and total CREB present in each sample.
Equal amounts of protein from each fraction were analysed by SDS-PAGE and western blott, and protein bands on X-ray film were quantified by densitometric scanning using a Bio-Rad Imaging densitometer.
Band intensities were measured by densitometric scanning using a densitometer and NIH Image 1.61/ppc software.
The absorbance was measured by densitometric scanning using the ImageJ software (http://rsb.info.nih.gov/ij/).
Gene expression was quantified by densitometric scanning using an Image Analyzer Quantity One Installer (Fuji Film Japan).
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The resulting blots were digitized and subjected to densitometric scanning using a standard NIH image program.
Optical density measurements were obtained with densitometric scanning using the LAS3000 (Fuji Film, Tokyo, Japan).
Immunoblot signals were visualized by an ECL chemiluminescence substrate reagent kit (Pierce) and were quantified by densitometric scanning and image analysis using Quantity One software Bio-Rad Laboratoriess, Hercules, CA).
Then, lipids were quantified by densitometric scanning at 400 nm using a Shimadzu dual wavelength chromatoscanner CS-930 with triolein as standard.
Quantification of ergosteryl esters was accomplished by densitometric scanning at 275 nm using a Shimadzu CS-930 dual-wavelength chromatoscanner and ergosterol (Sigma) as a standard.
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