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Intensity of immunoreactive bands was estimated by densitometric quantification using the Gel analyzer v1.0 software.
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Following staining with CBB (Coomassie Brilliant Blue R-250), the amount of product was determined by densitometric quantification of the 17 kDa band using the GS-710 scanning densitometer (Bio-Rad Lanalysedies) and analysed using the 1D Phoretix Software (Nonlinear Dynamics).
Following SDS/PAGE (10% gel) and staining with Coomassie Brilliant Blue R-250, the amount of product was determined by densitometric quantification of the 35-kDa band using the GS-710 scanning densitometer (Bio-Rad Lanalysedies) and analysed using the 1D Phoretix Software (Nonlinear Dynamics).
(d) Percentual distribution of OmpA, Stx2a, ShET1 and H4 flagellin in OptiPrep gradient fractions 1 to 7 as demonstrated by densitometric quantification of immunoblot signals shown in panel (b) using Quantity One® (the sum of signals of each virulence factor in fractions 1 to 7 was considered 100%).
Gelatinolytic activity was determined by densitometric quantification of the substrate lysis zones around 92 (pro-MMP-9) and 72 (pro-MMP-2) kDa using the ImageJ analysis software [ 31].
The protein levels were evaluated by densitometric quantification in reference to the GFAP immunostained area in five representative pictures taken from the neocortex of each animal using Adobe® Photoshop® CS4.
To control for input in Beclin 1 anti-immunoprecipitates, values used to calculate VPS34 lipid kinase activity were normalized for levels of Beclin 1 determined by densitometric quantification of Beclin 1 western blot bands in anti-Beclin 1 immunoprecipitates.
The initial rates of the reaction are determined by densitometric quantification of the product 1,2-diacylglycerol after its separation by high-performance TLC and staining with a CuSO4/H3PO4 or p-methoxybenzaldehyde/H2SO4 reagent.
The determination of signal intensities resulting from the amplification reactions was done by densitometric quantification.
By densitometric quantification, a mean 2.0-fold2.0-folde in p53 induction was present (range: 1.3 3.2; P=0.0038).
Protein abundance was determined by densitometric quantification of the protein spots on 2D-electophoresis gel followed by MALDI-TOF mass spectrometry.
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