Sentence examples for by densitometric quantification of from inspiring English sources
The initial rates of the reaction are determined by densitometric quantification of the product 1,2-diacylglycerol after its separation by high-performance TLC and staining with a CuSO4/H3PO4 or p-methoxybenzaldehyde/H2SO4 reagent.
(d) Percentual distribution of OmpA, Stx2a, ShET1 and H4 flagellin in OptiPrep gradient fractions 1 to 7 as demonstrated by densitometric quantification of immunoblot signals shown in panel (b) using Quantity One® (the sum of signals of each virulence factor in fractions 1 to 7 was considered 100%).
Protein abundance was determined by densitometric quantification of the protein spots on 2D-electophoresis gel followed by MALDI-TOF mass spectrometry.
To control for input in Beclin 1 anti-immunoprecipitates, values used to calculate VPS34 lipid kinase activity were normalized for levels of Beclin 1 determined by densitometric quantification of Beclin 1 western blot bands in anti-Beclin 1 immunoprecipitates.
Gelatinolytic activity was determined by densitometric quantification of the substrate lysis zones around 92 (pro-MMP-9) and 72 (pro-MMP-2) kDa using the ImageJ analysis software [ 31].
We found significantly increased expression of SAMHD1 in lysates of control fibroblasts treated with Poly(I C) i.e., a 1.5 to 2.2-fold induction as revealed by densitometric quantification of SAMHD1 expression normalized to β-actin (data not shown), while the amount of SAMHD1 protein was not changed or only very slightly up-regulated after treatment with TNF-α.
Western blot analysis of four whole-brain lysates per genotype followed by densitometric quantification revealed that the levels of GFPu were not altered in R6/2 mice (Figure 1B), in agreement with lack of gross proteasome inhibition in end-stage R6/2 brains [12].
Intensity of immunoreactive bands was estimated by densitometric quantification using the Gel analyzer v1.0 software.
The determination of signal intensities resulting from the amplification reactions was done by densitometric quantification.
By densitometric quantification, a mean 2.0-fold2.0-folde in p53 induction was present (range: 1.3 3.2; P=0.0038).
The protein levels were evaluated by densitometric quantification in reference to the GFAP immunostained area in five representative pictures taken from the neocortex of each animal using Adobe® Photoshop® CS4.
