The band intensity was semi-quantitated by densitometric analysis using ImageJ software.
Protein bands were quantified by densitometric analysis using Image J software 1.46 (National Institute of Health, USA).
(F) The optical density of M protein band in Fig. 8E was measured by densitometric analysis using ImageJ software and then the ratio of M protein/β-actin was calculated.
Bands corresponding to protein expression of AQP5, α-ENaC, α1-Na,K-ATPase, NKCC1, TLR4, and actin were quantified by densitometric analysis using Image J software (Research Services Branch, National Institutes of Health, Bethesda, MD, USA).
Differential changes in cytokine production were calculated by densitometric analysis using ImageJ 1.42 (NIH) software.
Alternatively, autoradiographs were scanned and quantitated by densitometric analysis, using the VersaDoc image analyzer and the Quantity One program (BioRad).
The changes in signal or band intensity were quantified by densitometric analysis using the Quantity One 4.4.1 program (Bio-Rad chemi-doc).
Results were quantified by densitometric analysis using ImageJ software, and were expressed as the OD for ratio to each GAPDH expression level.
Protein quantification for each sample was performed by densitometric analysis using KODAK imaging software and normalized to actin in the same sample.
The chemiluminescence ECL reactive (Enhanced Chemiluminescent System, Amersham Biosciences) was used to develop the bands, which were analyzed by densitometric analysis using the Gel Doc-Quantity One 4.5.0.
The intensities of the SA reacted and non-reacted HC bands were determined by densitometric analysis using 1D Gel Analysis software (Kodak Digital Science).
