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The PfRH5 protein immunogen was pure and shown to be correctly folded by demonstration of binding to its receptor, basigin (Crosnier et al., 2011).
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Methicillin-resistance was confirmed by demonstration of penicillin binding protein 2a with a latex agglutination antibody screening kit (Denka Seinken Co. Ltd ,Tokyo, Japan).
3, 6, 7 These neuronal- or glial-surface directed antibodies 8 are detected by demonstration of antibody bindinglial-surface directedmantibodiesic kidney cells that have been made to express the specific target (Fig 1A–C), to the surface of cultured live neurons (Fig 1D), and to fixed brain sections (see Fig 1E; these techniques have different merits and will be discussed later).
Specificity of the vitronectin antibody was confirmed by ELISA demonstration of binding to purified recombinant vitronectin, but not fibronectin or collagen (not shown).
Assembly of functional Fab was confirmed by demonstration of antigen-specific binding using antibodies of known specificity.
Whilst such approaches do not necessarily require the demonstration of binding legal or corporeal effects, they nonetheless often have forms of conditionality that will limit their application.
Diagnosis is confirmed by demonstration of the infarcted areas by computed tomography (CT) scanning.
A VSD may be identified either morphologically or by demonstration of a shunt.
Diagnosis was confirmed by demonstration of Leishmania amastigotes by microscopy and/or demonstration of Leishmania DNA with PCR/qPCR test as reported earlier [ 14, 15].
Biochemical confirmation of apoptosis was obtained by demonstration of PARP cleavage by Western blotting.
Diagnosis of toxoplasmosis by demonstration of T. gondii is usually difficult.
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