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Expressed sequence reads were generated by deep transcriptome sequencing from two sets of normalized cDNA libraries.
MiRNA-associated targets are then identified and quantified by deep transcriptome sequencing.
This is the first report of development and validation of a comprehensive set of genic-SSR markers in pigeonpea by deep transcriptome sequencing using next generation sequencing technology.
In this study we focused on transcripts that were discovered by deep transcriptome sequencing and were not yet annotated in the current bovine genome assembly.
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Here we report the discovery of genetic variation in Miscanthus sinensis using SNP markers discovered by both deep transcriptome sequencing and amplification of SSRs that were previously shown to be variable in sugarcane.
In the assembly, these ESTs were distributed in 4,616 unigenes, among which 962 (20.8%) were not captured by our 454 deep transcriptome sequencing.
We developed 550 validated genic-SSR markers in pigeonpea using deep transcriptome sequencing.
A further deep transcriptome sequencing using staged meiosis-I meiocytes is currently being performed.
It is likely that with deep transcriptome sequencing more fungal AS events will be found.
Efforts are underway to produce deep transcriptome sequencing coverage of multiple Silene species.
L. luteus deep transcriptome sequencing will facilitate the further development of genomic tools and lupin germplasm.
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