Sentence examples for by deep sequencing qPCR from inspiring English sources

To validate the expression levels of the known miRNA and mRNA genes determined by deep sequencing, qPCR primers were selectively designed for six miRNA and six mRNA genes.

These results suggested that the vast majority of HLH-1 bound intervals detected in embryonic chromatin by array hybridizations are factor-specific and are reproducibly detected by deep sequencing or qPCR, with the confidence of detection directly related to the peak score.

We employed quantitative TaqMan PCR (qPCR) to validate miRNA expression determined by deep sequencing.

After RT-qPCR amplification, they were confirmed to have expression patterns similar to those observed by deep sequencing.

Composition of the microbiota was explored by deep sequencing.

dPutative sizes of the sRNAs detected by deep sequencing.

These results and those obtained by deep sequencing (P.

Bi-allelic mutations were confirmed by deep sequencing.

The cDNA library was then analyzed for coverage by deep sequencing using a Hiseq2000 sequencing platform.

RNA was then harvested, processed (see above) and analysed by deep sequencing using the Illumina platform.

In addition, the sequence of several new MDV-encoded microRNAs were discovered by deeper sequencing.