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Indel analysis on the rhPCSK9 targeted locus by deep sequencing of PCR amplicons.
Sultan, M. et al. A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome.
Van Tassell, C. P. et al. SNP discovery and allele frequency estimation by deep sequencing of reduced representation libraries.
Vaz, C. et al. Analysis of microRNA transcriptome by deep sequencing of small RNA libraries of peripheral blood.
The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments.
shRNAs that affect cellular sensitivity to a given compound can be identified by deep sequencing of shRNA-specific barcodes.
Hirakawa, H. et al Dissection of the octoploid strawberry genome by deep sequencing of the genomes of fragaria species.
Kostic, A.D. et al. PathSeq: software to identify or discover microbes by deep sequencing of human tissue.
Here we describe methods for the large-scale analysis of genetic variation in Plasmodium falciparum by deep sequencing of parasite DNA obtained from the blood of patients with malaria, either directly or after short-term culture.
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Although mutually-exclusive var gene expression in ring stages 14 hours post-invasion (hpi) was unaltered in the mutants, upregulation of members of other clonally variant gene families and putative exported proteins was observed by deep-sequencing of the transcriptome of mutant parasites in late stages (30 hpi).
The method is based on the co-immunoprecipitation of DNA binding proteins with formaldehyde cross-linked DNA, followed by deep-sequencing of the immunoprecipitated chromatin fragments.
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