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This study represents the first comprehensive survey of miRNAs expressed in skin of animals of different coat colors by deep sequencing analysis.
By deep sequencing analysis of five favourable and five unfavourable MYCN-amplified neuroblastoma, Schulte et al (2010) reported the underexpression of 20 miRNAs.
The aims of this study were to identify and characterize miRNA genes in Atlantic salmon by deep sequencing analysis of small RNA libraries from nine different tissues.
Validation of this micro-array experiment was performed by deep sequencing analysis of RNA samples derived of 4 batches of approximately 150 embryos at the 5 day time point of infection and 4 non-infected batches of embryos.
Based on the RegulonDB database and the results of this study, the longer transcripts may be expressed from novel TSSs (i.e., each transcript detected by deep sequencing analysis was part of a 5'-UTR, not an sRNA).
To better understand the potential role of miRNAs in the post-transcriptional regulation of genes linked to pigmentation, we have characterized the miRNA transcriptome in skin of alpaca of different coat colors by deep sequencing analysis.
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Given the impact of nbr on miRNAs, we also analyzed the effect of nbr on piRNA and endo-siRNA lengths by deep-sequence analysis of libraries from ovaries.
To investigate whether mRNA expression was also changed by AA treatments, the 8 rat kidney samples were analyzed by mRNA deep sequencing analysis in parallel.
The identification of brain-related miRNAs by our deep sequencing analysis shows that the dataset is reliable not only for characterising expression profiles of known miRNAs but also for discovery of novel miRNAs.
The identification of FNS-related miRNAs by our deep sequencing analysis shows that the dataset is reliable not only for characterizing expression profiles of known miRNAs but also for discovery of novel miRNAs.
Future work should investigate any potential divergence in viral characteristics by phylogenetic and deep sequencing analysis between cases detected by various different routes that is, routine surveillance for influenza-like illness versus direct clinical presentation otherwise.
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