Sentence examples for by decreasing transcript levels from inspiring English sources
MicroRNAs (miRNAs) are ∼22 nucleotides long non-coding RNAs that in mammals predominantly act as post-transcriptional regulators and affect gene regulation by decreasing transcript levels mainly through the degradation of mRNA [1], [2].
Previously, we showed that adaptive substitutions in one of the three promoters of the bacteriophage ϕX174 improved fitness at high-temperature by decreasing transcript levels three- to four-fold.
A significant reduction in galactolipid content was found in the s6k1, raptor2 mutant or TOR-inhibited plants, which was accompanied by decreased transcript levels of the set of genes such as lipid phosphate phosphatase α5 (LPPα5), MGDG synthase 1 (MGD1), and DGDG synthase 1 (DGD1) involved in galactolipid synthesis, compared to the control plants.
Although recent studies demonstrate that repression of proteins is frequently mirrored by decreased transcript levels of miRNA targets [15] [17], examples where translational repression is the major component of silencing have been identified as well [17] [19].
The physiological mechanism by which decreased transcript levels may lead to increased fitness is still to be ascertained.
While knockdown efficiency ranged from 20to90%0%, most RNAi lines decreased transcript levels by 40% or more.
Likewise, nonsense mutations in the coding sequence of a gene can decrease transcript levels by nonsense-mediated decay [ 22].
These decreases were supported by toxicogenomic analyses, which showed decreased transcript levels for TNFα and IL-1β at ≥ 60 mg/l SDD (Kopec et al., 2012a).
Importantly, none of the genes encoding enzymes of ergosterol biosynthesis or ABC transporters exhibited decreased transcript levels by the starvation stresses.
Evaluation of microarray data by qRT-PCR confirmed considerably decreased transcript levels of hpp1 in Δ phlp1 and co-regulation of hpp1 with ste6 in Δ phlp1 and Δ gng1.
Over the entire period studied (day 8 35) we did not observe decreased transcript levels for vimentin as claimed by other investigators [22].
