Sentence examples for by decreasing the affinity from inspiring English sources

CTP inhibits the CTP synthetase activity by increasing the positive cooperativity of the enzyme for UTP, and at the same time by decreasing the affinity for UTP (Yang et al. 1994; Nadkarni et al. 1995).

This phosphorylation inactivates EEF2 resulting in termination of peptide elongation by decreasing the affinity of the elongation factor toward the ribosome., Other stimuli such as stress and growth factors that promote protein synthesis must inhibit the activity of EEF2K.

Glucokinase regulatory protein (GCKR) is expressed in liver and, to a lesser extent, in β-cells and exerts an inhibitory effect on glucokinase (GCK) by decreasing the affinity of the latter for glucose (1).

The CD14 C-159 T polymorphism involves a C → T nucleotide substitution, located −260 bp from the translation start site and −159 bp from the transcription start site, which alters CD14 promoter activity in vitro by decreasing the affinity of Sp protein binding and thus enhancing transcriptional activity [ 19].

Thus, we hypothesized that background could be reduced by decreasing the affinity with which TEV binds its cleavage sequence (i.e., increasing KM) and/or by reducing the rate at which cleavage occurs once TEV has bound its cleavage sequence (i.e., decreasing kcat), such that the probability of trans-cleavage occurring during a transient encounter is reduced.

Our results establish that in the background of Q151Mc, K70Q causes TFV resistance through a third mechanism: by decreasing the binding affinity of the inhibitor (Kd-dependent exclusion mechanism).

In keeping with this, some strains of H. pylori may have evolved to either inactivate apo-Fur regulation of sodB, or to weaken repression by decreasing the Fur/sodB binding affinity.

This would generate different afterhyperpolarization depending on the length of the repeat, and ultimately result in increased excitability, as shown for dopaminergic neurons, where decreasing the activity of SK channels by decreasing the apparent Ca2+ affinity changes the firing pattern from a pacemaker to an irregular or bursting one (Ji et al, 2009).

Klein et al. show that somatic mutations in the immunoglobulin framework region of antibodies against HIV-1 enhance affinity by decreasing the dissociation rate and are generally necessary to achieve broad neutralization31.

Furthermore, the calculation of Kb for clozapine demonstrates that the intracellularly located point mutation affects the extracellular binding for clozapine via two mechanisms: either directly by increasing the binding affinity and/or indirectly by decreasing the EC50 for 5-HT.

Dam1 is phosphorylated by Ipl1/Aurora B, which decreases the affinity of the Dam1 complex for microtubules (Gestaut et al. 2008), but Duo1 is not known to be a substrate for Ipl1.