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Protein identifications were achieved by database comparisons using search algorithms such as Mascot [22] or MS-FIT http://prospector.ucsf.edu, whereby Mascot was available as in-house version.
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All database comparisons used Microsoft Access 2007.
Annotation of COG and KEGG pathways was performed by sequence comparisons using BLASTX algorithm against Cluster of Orthologous Groups database and Kyoto Encyclopedia of Genes and Genomes database with an E-value threshold of 10-5.
Sensitivity is improved by profile profile comparison, using profiles for every database entry (Soding, 2005).
The templates for the protein models were identified by HMM-HMM comparison using the pdb70_7Mar09 Hidden Markov Model (HMM) database 23 via the HHpred server.
Genomic gene content and NCBI non-redundant sequence database comparisons were performed using BLAST [121].
Database comparisons are typically performed using peptide mass fingerprinting [ 19, 20], and quantization is performed by gel image intensity evaluation or by protein tagging [ 21, 22].
Database comparison was performed using ProteinCenter, v3.2.0.9 (Proxeon, Odense, Denmark).
Identification of 16S rRNA genes was done by comparison with EMBL/Genebank/DDBJ database and RDP database using BLASTN and Classifier, respectively.
Sequences were first sorted by using a subtractive database comparison procedure.
The database comparisons were performed by BE.
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