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Donor mouse blood in citrate buffer was pooled and bacterial concentration determined by darkfield microscopy using a Petroff-Hausser counting chamber.
B. burgdorferi were enumerated by darkfield microscopy using a Petroff-Hausser chamber.
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Stained sections were examined using both brightfield and darkfield microscopy using a Zeiss Axioskop II with attached charge-coupled device camera (Carl Zeiss Microscopy, Hertfordshire, UK).
Direct observation of leptospires in blood samples by darkfield microscopy is notoriously unreliable and not recommended [2].
Incubation was done at 30°C and cultures were inspected by darkfield microscopy for growth of leptospires at regular intervals up to 4 months.
Biopsies were cultured in BSK-H media containing rifampicin (50 ng/ml) and phosphomycin (100 ng/ml) and were examined for the presence of B. burgdorferi by darkfield microscopy as previously described.
Cultures were incubated for 6 weeks at 35°C and examined weekly for spirochetes by darkfield microscopy.
On receipt at the laboratory, fresh human blood samples were examined by darkfield microscopy for viable Borrelia and, if detected, 200 μL of blood was injected into 10-mL vials of BSK-H medium (14 ) and into 10-week-old ICR mice by the intraperitoneal route.
QUM analysis examines the organization and integrity of sperm organelles by electron microscopy, using both transmission electron microscopy and scanning electron microscopy.
Using abrasive slurries with micron and sub-micron particulates results in particle adherence to the surface, as can be readily observed using Darkfield microscopy.
To confirm loading, T cells were imaged using darkfield microscopy.
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