Sentence examples for by cycle sequencing using from inspiring English sources
The nucleotide sequence determination was carried out by cycle sequencing using an ABI 3730xl DNA sequencer (GATC Biotech AG, Konstanz, Germany).
Sequencing was carried out on PCR-amplified cDNA segments by cycle sequencing using Thermo Sequenase enzyme.
DNA sequencing was conducted by cycle sequencing, using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Darmstadt, Germany) according to the manufacturer's instructions.
followed by cycle sequencing using the Big Dye® Terminator v.3.1 kit (Applied Biosystems®, Darmstadt, Germany), and a final purification using the Big Dye® XTerminator™ kit (Applied Biosystems®).
Miniprep DNA of >5 cDNA clones from each viral genome segment was sequenced by cycle sequencing using IRD800 and IRD700 infrared dye labeled M13 (Eurofins MWG Operon, Ebersberg, Germany) and TOV segment 2 specific internal primers.
The nucleotide sequences of the amplified fragments were confirmed by cycle sequencing using a DYEnamic ET dye terminator cycle sequencing kit (MegaBACE; Amersham Pharmacia Biotech, Schenectady, NY, USA) and MegaBACE 1000 DNA sequencer (Amersham Pharmacia Biotech) (Mitsui et al, 2006; Hattori et al, 2009).
Sequences were obtained by standard cycle sequencing using an ABI 373 sequencer (PE Applied Biosystems).
The pol coding region from the recombinant viruses was confirmed to be identical to their respective parental virus pol sequences by terminator cycle sequencing using an automated model 377 DNA sequencer (Perkin Elmer, Applied Biosystems).
Sequence alterations were identified by direct cycle sequencing using BigDye fluorescent terminators and the ABI sequencer (Applied Biosystems).
The variable antibody genes from two clones of each restriction pattern were analyzed by PCR cycle sequencing using infrared-labeled primers according to the manufacturer's instructions (Licor).
Purified PCR products were sequenced in both directions (primer sequences available upon request), by fluorescent cycle sequencing using the 'Big Dye® Terminator v3.1 Cycle Sequencing' kit (Applied Biosystems, Foster City, CA, USA) according to manufacturer's recommendations.
