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Routes are then obtained by cutting the sequence just at the point before problem constraints (capacity and time) are violated.
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In the route construction, when cutting the sequence, both capacity and time constraints are considered.
Thus, by cutting the short reads, the sequences of TR with long patterns are collapsed and can not form cycles in the de Bruijn graph.
Seventy-four representative bands from 31 lions were sequenced by cutting the band out of the gel and soaking in dH2O overnight, re-amplifying the DNA from the band using a new forward primer (Bab8: 5'ATT GGA GGG CAA GTC TGG 3') with Bab7 primer (459 bp).
Two different quasi-isotropic stacking sequences, obtained by cutting the specimens in two perpendicular directions, were also tested for each geometry, in order to study the influence of both these factors on the impact and post-impact response.
DGGE has advantages over other molecular approaches in that it is a high throughput, relatively inexpensive method that allows detailed characterisation of predominant bacteria by cutting the individual bands, re-amplifying and sequencing, without the need for a cloning procedure.
The coding sequence of the EGFP gene was removed by cutting the PKCδ plasmid with EcoRI and NotI restriction enzymes (Roche Diagnostics, GmbH, Mannheim, Germany).
More significantly, youth court saves by cutting the recidivism rate.
Spice it up by cutting the brake line.
Cuttings were prepared by cutting the hypocotyl 4 cm below the point of cotyledon attachment.
Then, reperfusion was performed by cutting the band with scissor.
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