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There is, however, another class of mutation that can be generated through SB transposition that is not detected by current sequencing methods.
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With current sequencing methods, we can sample genomes comprehensively.
The emerging picture from the conference revealed that the kinds of information supplied by current genome sequencing methods have surpassed the existing theory and methods for evolutionary reconstruction from molecular data.
Unfortunately, the current WTA method generates primer sequences on both ends of the amplified cDNA fragments and these sequences would be read first when analyzed by current massive parallel sequencing methods.
Our TILLING population was treated with 0.4% EMS for 12 hours, and compared with the previously reported mutation frequency of 1 SNP/1,067 kb for single copy genes for the same population, the current TILLING by sequencing method had a higher chance for mutation detection [ 40].
Current sequence-based methods could be made much less arbitrary by comparing them against these other lines of data.
All constructs were verified by standard sequencing methods.
Nucleotide sequences of cDNA clones were determined using automated DNA sequencing machines by standard sequencing methods.
This observation imposes a great challenge to precisely predict protein solubility from amino acid sequences by current bioinformatics methods.
Targeted NGS can reveal sequence changes undetectable by current diagnostic methods.
Not only does whole genome sequencing provide the inter-cluster differentiation provided by current typing methods, but it also achieves intra-cluster resolution which can be used to inform epidemiological investigation.
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