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Action potentials were stimulated by current injection of +70 to +220 pA.
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(A ) Representative traces of responses elicited by current injections of −150, −50, 0, +50, and +300 pA in WT and Rgs7 −/− CA1 neurons.
Action potentials were recorded by current injection to maintain an initial holding potential of approximately −70 mV and then a hyperpolarizing current step was delivered (−40 pA), with subsequent 30 pA depolarizing steps up to 100 pA at a rate of 1 Hz up to seven repetitions.
The sections were observed under a light microscope, and the recording sites were reconstructed according to the lesions made by current injection and traces of electrode tracks.
The main source for this sort of noise is probably at the level of synaptic transmission[45], since neurons stimulated directly by current injection in the absence of synaptic input give highly stereotyped and precise responses[46].
AHP was induced after a burst that was evoked by a current injection of depolarizing step into soma (200 ms duration).
A burst of spikes artificially evoked in PeD12 by current injection resulted in a single large contraction of the columellar muscle, which is known to cause touch-induced whole-body withdrawal responses [ 4].
We confirmed that when NVmes cells are depolarized by current injection, firing occurs during the depolarizing crests of the oscillations [19].
For calculating the time course of membrane potential changes relative to the pairing spike, we calculated the time of each neuron's pairing spikes relative to the stimulus as the maximum of a Gaussian filtered (σ = 10 ms) peristimulus time histogram (PSTH) of APs evoked by current injection during pairing.
Fig. S5 shows membrane properties of photoreceptors at different illumination and temperatures as pooled statistics of five photoreceptors, studied by current injection experiments.
(G) Representative action potential responses of a hiPSC-derived neuron evoked by current injection (Bars: 100 ms and 20 mV).
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