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In reality, as cyclists are slowed down by crossings for pedestrians and detours behind bus stops, they require a more careful pace.
Constitutive Sp2null and conditional Sp2cko knockout alleles were obtained by crossings with appropriate Cre recombinase expressing mice.
Experimental and control animals were obtained by crossings of heterozygous mutants with C57BL/6 mice.
The quadruple mutant lro1Δdga1Δare1Δare2Δ of the budding yeast S. cerevisiae was obtained by crossings and tetrad dissection of the respective single deletion strains (EUROSCARF strain collection).
More than 400 males and females were checked by crossings with scattered or heterozygous scaled spawners in the third and fourth generations.
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APP/E2 and APP/E4 mice were generated by crossing-breeding of APPSW/PS1dE9 mice [ 14] with APOE ε2-TR or APOE ε4-TR mice [ 16, 17] as previously described [ 13].
The 10 highest-ranked sequences proceeded to the next round of the algorithm without modification, while 90 new mRNA sequences were generated by crossing-over or mutating randomly selected sequences from the 100 original mRNA sequences.
Activity, as measured by center crossings, and grooming were also reduced by subchronic treatment with fluoxetine.
Discounts of up to 20percentt are available for bookings made by March 31 for 2002 crossings.
To the south is genuine wilderness, with the main road interrupted by river crossings.
The frisson created by border crossings is an emotional Doppler effect: mounting anticipation, slowly easing on the other side.
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