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Here, we investigated the function of Sept4 proteins during mouse development by creating a targeted deletion of the Sept4 genomic locus.
We have shown that indeed the sap gene is also involved in phage resistance and most likely is the receptor of AP50c phage by creating a targeted deletion of this gene and showing that the Δ sap strain is phage resistant (manuscript in preparation).
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First they tested it by creating a target tone with a much higher ultrasonic frequency.
A BAC clone spanning the first 2 exons of Visc-1 was modified by recombineering and subsequently subcloned to create a targeting construct (Fig. 3 A, B).
"But the risk is that the presence of armed forces places the teachers at further risk of attack by creating a military target".
Among the coalition of artists denouncing Donald Trump, the White Stripes went one step further this week by creating a T-shirt targeting the Republican nominee.
By creating an inequality target that doesn't involve the top end – the rich – the SDG target actually allows room for greater income concentration at the top.
A similar effect can be achieved by creating a partial inverse that targets key areas of the face.
3. Explain to students that they will be collaboratively creating informational materials about some summer hazards by creating a public service announcement (PSA) targeted to young people about a particular summer hazard.
To delete the +3.5 G4 motif sequence from BU-1, we created a targeting construct by amplifying a genomic region between exon 1 and exon 4 with primers BU1SalF and BU1NotR (for oligo sequences see Supplementary Table S1).
Conceptually, multi-target drugs work by creating a combination effect on multiple targets in the biological network simultaneously, which may (through e.g. synergistic effects) decrease the therapeutic dose required, thus increasing therapeutic efficacy, preventing drug resistance, and reducing target-related adverse effects [12 14].
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