Topo II α reduces DNA supercoiling and twisting by creating a double-strand nick that enables the passage of a second DNA double-strand through the break and subsequent religation of the cleaved DNA strand.
They function by creating a transient double-strand break in one double helix and passing a separate intact DNA segment through the opening.
In the eyes of his critics, he is creating a double standard by legitimizing an Indian weapons program that only eight years ago led Washington to impose huge sanctions, while demanding, in the same week, that Iran and North Korea give up any capacity to make their own nuclear fuel.
Fox takes the process one step further by creating a threat from whole cloth, then doubles down on their deceit by converting an actual good into something undesirable.
Double pane windows, made by creating a vacuum between two panes of glass, are more energy-efficient but tend to fail faster when exposed to high temperatures.
Instead of pairing Puccini's comic "Gianni Schicchi" with one of its somber siblings from "Il Trittico" ("The Triptych"), Juilliard Opera programmed it with "L'Heure Espagnole" ("The Spanish Hour") by Ravel to create a double bill of amorous and avaricious lust.
By creating an independent bond insurer with a double-A rating, MBIA should give an immediate lift to roughly $550 billion in outstanding municipal bonds, which had all lost the triple-A ratings they previously had been able to purchase when they bought insurance from MBIA.
But Marlins Manager Tony Perez runs out from his dugout and seems to persuade the umpires that when Johnson tagged both Zeile and Ordóñez, it was Ordóñez who was out, under Rule 7.03, and that Zeile had created a double play by then mistakenly leaving the base.
- She creates a double negative by crossing out the terms "not baked" and "not fried".
This is the second time in her career that Whiteread has filled an accidental artistic absence: for the fourth plinth in London's Trafalgar Square, she created a double-take by casting a resin replica of the plinth itself.
