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The role of membrane-bound Dlk1 in skeletal muscle was investigated by creating a conditional mutant mouse model.
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By creating a new mouse model with conditional gene deletion of Lkb1in the epithelium of the small intestine we have shown that Lkb1 is necessary for normal intestinal cell differentiation and maturation.
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In addition, we created a conditional auxotroph by repressing synthesis of the carboxysome, an essential component of the carbon concentrating mechanism cyanobacteria use to fix atmospheric CO2.
We reasoned that it should be possible to create a conditional C-degron by constructing a tag with the cODC degron kept inactive due to a protective peptide added C-terminally to the cODC degron.
In recent experiments, we created a conditional Shp2 knockout (Shp2flox) allele in mice by inserting two loxP sites into introns that flank exon 4 of Shp2.
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