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Total cell number was determined by counting each sample in duplicate using a hemocytometer under an inverted phase contrast microscope (Olympus Corporation, Tokyo, Japan) using Trypan blue dye.
Total cell number was determined by counting each sample in duplicate using a hemocytometer under an inverted phase contrast microscope (Carl Zeiss) using trypan blue dye.
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The concentration of nuclei was estimated by counting a sample in a hemocytometer and adjusted to ∼300 400 nuclei per μl.
The mean number of cysts per brain was determined by observation under an optical microscope, by counting four samples of 20 μl aliquots of each homogenized brain.
The mean number of cysts per brain was determined by counting three samples of 10 μl aliquots of each homogenized brain under an optical microscope.
By counting wood samples, the analysis also created a rough measure of human activity.
Classification of the incoming pixel is done by counting the samples in a fixed-size window around it, with the threshold being 2 samples out of a set of 20.
The percentage of macrophages infected with parasites was determined by counting 4 samples of 100 cells.
This technique directly yields the picosecond time-resolved fluorescence decay for every pixel by counting and sampling single emitted photons.
We also calculated cumulative present calls by counting the samples in which a given probe had been called present.
Osada evaluated N samples from shape distribution and constructed a histogram by counting how many samples fall into each of the B-fixed sized bins.
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