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This study attempts to discuss whether it is possible by a simple procedure to distinguish between very likely endogenous and uncertain endogenous CK's by counting and evaluating all the human CK's identified by mass spectrometry in a human and murine study.
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Cumulative risk was assessed by counting risk alleles and evaluating the difference in cumulative risk scores between case subjects and control subjects.
On day 9 adherent cells were detached, and counted and then evaluated by flow cytometry (30% of cells were CD11c positive and 65% were F4/80 positive).
The ratio of radiolabeled nucleotide incorporation was evaluated by counting before and after TCA precipitation, and was systematically close to 80% [ 55].
After 8 h at 37°C in a 5% CO2 incubator, cells that had attached but not migrated were scraped from the upper surface, membranes were fixed in 70% ethanol at −20°C and the migrated cells were stained with haematoxylin and eosin and evaluated by counting cell nuclei in 10 randomly chosen fields under light microscopy.
Fluorescence levels were assessed with excitation/emission at 485 nm/505 nm for GFP and 550 nm/570 nm for CF 555 using a BX53 fluorescent microscope with 100-w mercury lamp unit (Olympus, Tokyo, Japan), photographed ( × 400), and counted by viewing the monitor and evaluating cell shapes or CD45 staining.
After incubation for 6 h at 37°C in a tissue-culture incubator, duplicate membranes were processed and evaluated by counting migrated cells on the underside of the membrane in 10 random fields under high power (×400) light microscopy [ 28].
The radioactive bands were excised and the radioactivity was quantified by scintillation counting to evaluate the incorporation of [1-C]OA into lipids.
The number of migrated and positive stained adhered colonies were quantified and evaluated manually by counting all colonies under a light microscope (Zeiss, Germany) and visualized.
As above, the growth fraction by p53-protein staining was evaluated by counting 500 consecutive cells and LI was made.
Cell viability was evaluated by counting trypan blue-positive and -negative cells under a phase-contrast microscope (Zeiss Axiovert, Aalen, Germany).
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