Incubating biotin-ODNR01 with CD4+ T cells, followed by binding whole-cell lysate onto avidin-coated agarose beads, showed increased binding of STAT1/3/4, but not of STAT5/6 in the eluate from the avidin beads, as detected by subsequent immunoblot analysis using corresponding anti-STAT antibodies (Fig 2D, left panels).
The theoretical T-RF values of the representative phylotypes represented in the clone library were calculated using the NEB cutter [36] and confirmed experimentally by T-RFLP analysis using the corresponding clones as templates.
We have derived the aa sequence of the corresponding ORFs and predicted the whole sequence architecture by computer analysis using the SMART program [ 34, 35]).
Subgroups corresponding to different gene copy numbers were defined by eye and confirmed by cluster analysis (using R statistical software version 2.5.0), and are delineated by elliptical lines.
Biological pathways identified by DAVID/EASE analysis using a list of 423 tissue-selective genes matched archetypal pathways in the corresponding human or mouse tissue.
The protein levels of phosphorylated eNOS (S1179), eNOS, eNOS dimers and GAPDH were determined by western blot analysis using specific antibodies against the corresponding proteins (Cell Signaling), respectively.
Drug responses and clinicopathological variables were related to the corresponding genotype in case control associations by cross-table analysis using Fisher's exact test with two-sided verification.
The corresponding PfPdxK-strep protein was further verified by western blot analysis, using a monoclonal antibody against the fused strep tag (Figure 5B).
Here, we could give the details of the corresponding analysis by using the Lax-Wendroff scheme.
Relative protein amounts were evaluated by a densitometric analysis using Image J software (La Jolla, CA, USA) and normalized to the corresponding Actin levels.
