The effective extensional viscosity has been ascertained by correction for the area of high molecular extension.
By correction for the detection efficiency of the gamma counter, the total number of disintegrations per cell was calculated.
The actual number of template molecules was calculated by the direct counting of the number of positive wells followed by correction for the Poisson distribution (File S1).
They are obtained from experimental KIEs by correction for the forward and reverse commitment to catalysis.
The change in protein level was determined by densitometric analysis of the immunoreactive bands by Quantity One software (BioRad) followed by correction for the respective loading control.
The percentage of cells expressing the different TGF-beta signaling proteins in murine cartilage was calculated by correction for the total number of cells present in the articular cartilage of the tibia.
The statistical package SPSS for Windows, Release 17.0, was used to compare normotensive and hypertensive groups collected at both 'peak' and 'trough' BP by one-way analysis of variance (ANOVA), followed by correction for multiple testing using the Bonferroni post-hoc test, to determine significance of differences between the groups.
Significant up- or down-regulation of the expression of the 13 genes (AP2A1, B4GALT1, C1orf63, CCR2, CFP, DIS3, NEAT1, NOXA1, OSM, PAPOLG, PRIC285, ZNF419, and BC037918) and the 6 proteins between healthy controls and patients with malignant disease (extra for FIGO I/II and FIGO III/IV patients) was assessed by t tests followed by correction for multiple testing by the Holm Bonferroni method.
Sources of spurious variance, along with their temporal derivatives, were removed through linear regression including 1) 6 parameters obtained by correction for rigid body head motion, 2) the signal averaged over the whole brain, 3) the signal averaged over the lateral ventricles, and 4) the signal averaged over a region centered in deep cerebral white matter.
Care was taken to remove differences in expression level from the data by correction for differences in proteasome expression level and by transformation of the data for removal of the differences in degree of expression of the individual probes.
We compared two methods that compensated for this signal with leaving the FRET ratio image uncorrected: 1. Pixel-by-pixel correction for the intensity of mRFP when directly excited; 2. the application of a threshold to the ratiometric image based on the genetic controls afforded by the pcnaK164R and usp1 cells.
