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Ovulation is induced by copulation.
The vast majority of turbellarians are hermaphrodites (have both female and male reproductive cells), and fertilize eggs internally by copulation.
Without ethanol, males actively courted females, which were typically followed by copulation (Figure 6B); however, the courtship activity was drastically diminished under the influence of ethanol on the first exposure (Figure 6A).
Indeed, precopulatory violent shaking by the male seems to increase the female's need for oxygen which is followed by copulation when the female is exhausted and non-resisting [30].
Neither testis length nor testis area was altered by copulation.
Neither testis length nor testis area appeared to be altered by copulation; no significant difference in testis length was observed between mated and unmated males in the 48 hours following copulation.
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What has only recently been discovered is that males in many species make subtle alterations to their ejaculates on a copulation-by-copulation basis, depending on their assessment of immediate sperm competition risk.
Microbrachius fossils that don't sport the grooved bones, presumed to be females, instead have dermal plates beneath the pelvis; ridges and bumps on the inside of those plates probably helped the females firmly hold the males' organ during side-by-side copulation (artist's representation above).
Furthermore, females lived longer when they copulated for longer, but only at 12°C (temperature by mean copulation duration and temperature by mean copulation duration by female size interactions in Table 2).
Females in estrus are followed by males, with copulation initiated by the female.
Rotifer life cycle does not allow such uncoupling, because the fate of a sexual rotifer female (being either male or diapausing-egg producer) is controlled by her copulation when young and thus by the density of males in the population.
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