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For the remaining 12 genomes, STs were determined by conventional sequencing methods (21).
We cannot exclude the possibility that additional specimens contained mixed viral populations with minor proportions of position-272 mutants not detectable by conventional sequencing methods.
Because PB2 (2,341 bp), PB1 (2,341 bp), and PA (2,233 bp) genes have very similar sizes, direct separation of each gene from co-infected samples is not possible by conventional sequencing methods.
Similar(57)
Thus, this study highlights the difficulty in traditional candidate gene approaches using conventional sequencing methods.
The sequences produced by random amplification and RPM version 1 were identical to those identified by the conventional sequencing method with the exception of ambiguous base calls (Ns).
Influenza B group 2 isolates were identified as B/New York/1/2002 strain (accession no. AF532565) by the conventional sequencing method.
Due to the complex nature of HLA genes, there are difficulties in elucidating complete HLA gene sequences especially HLA gene haplotype structures by the conventional sequencing method.
The observed mutation can also be verified by conventional sequencing.
However, these events are not detected by conventional DNA sequencing methods commonly utilized for tumor genotyping.
Therefore, their corresponding TUs escape detection by conventional sequence analysis.
Sequences obtained by the conventional Sanger sequencing methods, generally >600 bp in length, are much more useful but the throughput is too low and the cost is too high.
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