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El Feghaly, R. E., Stamm, J. E., Fritz, S. A. & Burnham, C. A. Presence of the bla(Z) beta-lactamase gene in isolates of Staphylococcus aureus that appear penicillin susceptible by conventional phenotypic methods.
We initially identified organisms by conventional phenotypic methods (10 ).
Traditionally, bacterial identification has been performed by conventional phenotypic methods [ 4, 10].
The plates were read daily for up to 4 days and isolates identified by conventional phenotypic methods.
For rifampicin resistance: similarly, although drug susceptibility testing by conventional phenotypic methods is not 100% accurate, it is considered to be the gold standard.
Comparing the 16S rRNA gene sequences of bacterial species is a useful approach for the identifying unusual clinical isolates or those which cannot be properly identified by conventional phenotypic methods (10, 11 ).
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Where speciation was reported, it was conducted in diagnostic laboratories using conventional phenotypic methods.
The most commonly used methods for AST are conventional phenotypic methods, based on culturing on agar (e.g. disk diffusion tests) or on microtitration plates (e.g. broth dilution tests) [ 3].
This assay showed 92.9% sensitivity and 91.8% specificity for detecting β-lactam resistant genes, relative to the conventional phenotypic method, and this assay correlated well with conventional quantitative culture counts.
Identification of the cultured isolate was done by conventional phenotypic and biochemical methods.
The plates were read daily for up to 72 hours and isolates were identified by conventional phenotypic and biochemical methods.
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