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A constant concentration gradient was obtained by continuous stirring of the media.
The encapsulation of DOX in CaCO3 nanocrystals was achieved by continuous stirring of the suspension overnight, in a dark environment at room temperature.
The encapsulation of Dox in CaCO3 nanocrystals was achieved by continuous stirring of the suspension mixture in the dark overnight at room temperature.
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The excess solvent was evaporated by continuous stirring for 45 min to 1 h followed by centrifugation at 10,500 rpm for 15 min to remove excess of aqueous solution.
The macerated sample has been taken with 250 mL of distilled water with a pH between 1.2 and 2.6 (adjusted with 0.5 M HCl), followed by continuous stirring for 20 min at 90°C.
The stoichiometric amount of the cellulose solution (0.5 g) and the POSS-amine (0.35 g) are dissolved in DMAc (50 ml) and stirred (300 rpm) for 1 h in the presence of terphthalic acid (0.5 g), followed by the continuous stirring of the mixture for another 2 h at 95 °C until the homogeneous solution is achieved.
It is followed by two times rinsing in PBS for a period of 24 hours each time by continuous stirring at 225 rpm [ 23, 24].
Commercial chitin (40 g) was weighed and taken in a beaker; 500 mL of concentrated hydrochloric acid was added, followed by continuous stirring at 4°C.
Mixing was conducted by continuous stirring with paddles in two different heights.
The temperature during the experiment was maintained at 30°C by continuous stirring.
was prepared by continuous stirring at room temperature.
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