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Quantification of DNA was achieved by constructing a standard curve of calculated Ct versus concentration for a set of DNA standards that were included in each run.
Primer-specific amplification efficiencies were determined by constructing a standard curve of serially diluted cDNA.
The efficiency of each primer set was first validated by constructing a standard curve through four serial dilutions.
The efficiency of each primer pair was determined by constructing a standard curve from serial dilutions (Table 4).
Quantification was obtained by constructing a standard curve from serial dilutions of normal genomic DNA (ref 11 691 112 001; Roche, Meylan, France).
The expression level of genes was determined by constructing a standard curve using serial dilutions of EDL933 genome DNA with known concentrations.
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A 10-fold dilution series of cDNA was included in each run to determine PCR efficiency by constructing a relative standard curve.
For example, students are tasked with determining the concentration of a substance by assaying known concentrations, constructing a standard curve, and comparing their unknown against the curve to determine its concentration (observation phase).
A 10-fold dilution series of cDNA was included in each run to determine PCR efficiency by constructing a relative quantification standard curve.
We tackled this problem by constructing a latent reference standard and then using it to develop and evaluate logistic regression and recursive partitioning models of administrative data variables.
Intuitively, NB filtering operates by constructing a density estimator using standard Naïve Bayes.
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by constructing a proper
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by constructing a user-friendly
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by constructing a composite
by constructing a majority-rule
by constructing a phylogenetic
by constructing a continuous
by constructing a functional
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by constructing a p53-specific
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by constructing a Polygenic
by constructing a morphological
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Justyna Jupowicz-Kozak
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