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We tested this further by constructing a phylogeny of sequences from organisms with more than one distinct sequence type.
By constructing a phylogeny of this family, we have identified and named a number of orthologous groups.
To place these studies in context, we began by constructing a phylogeny of the Bacillaceae using whole-genome single copy genes.
This result was verified by constructing a phylogeny using representative insect and crustacean protein sequences and by including additional Daphnia ferritin-like sequences that were extracted from an ongoing D. pulex cDNA sequencing project (Colbourne et al. in preparation).
The usefulness of the SSR assays for diversity analysis and taxonomic discrimination was demonstrated by constructing a phylogeny based on SSR polymorphisms, and by the demonstration that most were also functional when tested with template from tomato, pepper and potato.
Data were analysed by grouping isolates into clusters of identical genotypes and by organizing all genotypes present in the overall sample according to genetic relationships, i.e. by constructing a phylogeny.
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This might be investigated constructing a phylogeny of Euonymus and timing the two phylogenies using a molecular clock [e.g., 61].
Classifying genes and constructing a phylogeny are important for the functional analysis of a gene family.
While we cannot engage meaningfully here with the fraught topic of Partula species designations [ 52, 64, 68], we can address the issue of lineage persistence by constructing a time-calibrated phylogeny for our molecular dataset.
The phyletic relationship between D. noxia and other arthropod species [ 10, 28, 34, 56, 59, 60] was examined by constructing a maximum-likelihood phylogeny from concatenated alignments of 37 single-copy proteins unique to arthropods.
Independence of data from correlation due to phylogeny was tested by constructing a phylogenetic tree for the selected felids (Figure S1) from which standardised contrasts were calculated (Text S5; Table S1).
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