By constructing a series of deletion mutants fused with EYFP and fluorescence microscopy analysis, we identified an N-terminal arginine-rich functional NLS (PASTPTPPKR GRYVVEHPEY) in P-ICP22, and KR is the crucial aa for its nuclear localization.
Cat-2 is the C. elegans homolog of mammalian TH, and it is expressed in all DAergic neurons, as demonstrated by a GFP construct fused to the cat-2 promoter (Lints and Emmons 1999).
A "bait" is constructed by fusing a protein, such as P1 to BD, and a "prey" is constructed by fusing another protein, such as P2 to AD, and both fusions are coexpressed in the same reporter cell.
As a concept multistage vaccine, we constructed a polyepitope by fusing five HLA-DR3-restricted T-cell epitopes derived from different Mtb proteins either expressed highly by replicating bacteria (Ag85B, hsp65, 19 kDa lipoprotein), or abundantly expressed by dormant bacilli and recognized preferentially by TST+ individuals (hsp16, Rv1733c).
Double domain PAS (dPAS) were constructed by fusing a PYR/PYL/RCAR to a PP2C via a variable linker region.
A derivative of pHsh was constructed by fusing a signal peptide to xynA2 gene to facilitate export of the recombinant protein to the periplasm.
This probe was constructed by fusing a CFP (cyan fluorescent protein) and a YFP (yellow fluorescent protein) with a specialized linker containing the caspase-3 cleavage sequence: DEVD.
Gene targeting vectors were constructed by fusing a GAL4 DNA-binding domain with a epigenetic suppressor, including CpG DNA methylase Sss1, histone H3 lysine 27 methylase vSET domain, and Kruppel-associated suppression box (KRAB).
The inverse correlation between responsiveness of ECM patterning to altered F-actin dynamics and higher order oligomer state of TSPs was investigated definitively by use of a chimeric construct that fused the coiled-coil oligomerization domain from TSP5 with the C-terminal region of TSP 1.
This exploits a novel cell surface luciferase as reporter gene, constructed by fusing a synthetic, codon-optimized version of the Gaussia princeps luciferase gene to Candida albicans PGA59, which encodes a glycosylphosphatidyl inositol-linked cell wall protein [ 14].
