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This hypothesis could be tested genetically by constructing a bacterial strain with a tagged efp gene.
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Unsupervised principal component analysis (PCA) was performed to investigate chemical variations in the bacterial samples by constructing a cluster-based segregation model (Huang et al. 2004).
The performance of the model was assessed for each type of serious bacterial infection by constructing a receiver operating characteristic (ROC) curve.
In the article, authors show that AMPHORA is scalable and efficient in HPC environments by constructing a phylogenomic tree composed by 578 bacterial species and by assigning phylotypes to 18,607 markers of metagenomic data collected from the Sargasso Sea.
For the primary screen, we used a bioluminescence-based method by constructing a luciferase-expressing reporter M.tb strain which contains the entire bacterial Lux operon cloned in a mycobacterial integrative expression vector.
Bacterial cell density was determined by colony forming unit counts (CFU; described by Sussman et al. [12]) and by constructing a cell density calibration curve of absorbance (595 nn) vs. CFU (Fig. S2B).
They successfully constructed a bacterial community with effective cellulose degradation by mixing the above two strains.
In order to expedite this approach, we have constructed a bacterial artificial chromosome (BAC) clone of the MVA genome using the elegant method devised by A. Domi and B. Moss for Vaccinia virus [53], with slight modifications.
However, to take full advantage of the ability to reduce coverage in Sanger-based plastid genome sequencing would require the sequencing of pure plastid DNA, something that can only reliably be achieved at present by constructing whole-genome bacterial artificial chromosome (BAC) libraries and then strictly sequencing plastid DNA-containing clones.
By constructing and analyzing a large database of bacterial whole genomes, we have probed the evolution of Gln-tRNAGln synthesizing molecular machinery.
The approach includes making retrieval constructs by sequence- and ligation-independent cloning (SLIC) and retrieving the full-length 3′-UTR in one exon to the retrieval construct from a bacterial artificial chromosome (BAC) by recombineering to generate the final full-length 3′-UTR reporter construct for the gene of interest.
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