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Microarray data was collected by confocal scanning using the Roche NimbleGen MS200 Microarray Scanner at 2 μm pixel resolution (Roche NimbleGen, Inc., Madison, WI, USA).
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At the end of the experimental period, non-attached S. mutans cells were washed with PBS and biofilms were observed by confocal scanning laser microscopy using a TCS-SP5 microscope (Leica Microsystems GmbH, Wetzlar, Germany) with reflected laser light at 488 nm, as well as a DMI6000 B fluorescence microscope (Leica) and a 63× oil immersion objective.
Imaging by confocal scanning laser microscopy (CSLM) was conducted using a Zeiss LSM 510 Meta Confocal system, equipped with Zeiss Axioplan 200 motorized microscope and a 405 diode argon, red and green He/Ne lasers.
The retinal thickness can be measured or quantified by Optical Coherent Tomography (OCT), Confocal laser scanning using Heidelberg Retina Tomography II (HRT II) or Retinal Thickness Analyzer.
The slides were evaluated by sequential scanning using a confocal laser scanning microscope (TCS SP5, Leica, Bensheim, Germany).
Confocal images were acquired by sequential scanning using a two-frame Kalman filter and a z-separation of 1 µm.
The double-labeled cells were observed and analyzed by pseudo-confocal scanning microscopy using a Zeiss microscope equipped with the ApoTome system.
Confocal scanning methods used by others in the field appear far more resilient to such scatter, which probably explains differences in rodent image quality between our flood system and other recently published work [ 11– 13].
Fluorescence was visualized by confocal microscopy using the Zeiss Meta confocal scanning laser microscope with a magnification of 40x.
All images of double stained skin tissue were acquired by confocal microscopy using a laser scanning microscope (Leica TCS NT SP).
Herceptin-Alexa Fluor 680 accumulation in the tumor cells was further studied by confocal microscopy using Leica confocal laser scanning microscope TCS SP5 (Leica Microsystems, Germany).
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