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Coverslips were mounted onto the slides using Fluorsave antifade reagent (Calbiochem), followed by confocal analysis using a Nikon C1 microscope (IByME, UBA, Argentina).
When the CD8+ T cells were stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin (IM), the increased level of enzymatic activity of cathepsin L was confirmed by confocal analysis using Magic Red™ (Figure 4A).
To confirm or invalidate this hypothesis, human fibroblast cells were treated either with hPaf1 or scramble siRNAs, synchronized at the G1/S boundary, released for 30, 60, 120, and 280 min, and analyzed by confocal analysis using the HP180-12 antibody recognizing the hypophosphorylated Pol α.
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Cathepsin L activity of CD8+ T cells stimulated with PMA and ionomycin (IM) was assessed by confocal microscopic analysis using a Magic Red™ cathepsin detection kit.
This was confirmed by confocal analysis of the interaction of LF82 OMVs with Intestine-407 cells using anti-O83 to label OMVs.
The percentage of double-labeled cells (BrdU+/NeuN+, BrdU+/GFAP+) was determined by confocal laser microscopic analysis using a 60× oil objective.
To investigate the persistence of the antifungal activity of already used Au-MUA-NH-EstC surfaces, microbial viability after adherence was assayed after 1 month's storage at 4°C by yeast numerations on plates and confocal microscopy analysis, using the same protocols described previously.
The enhanced neuropil degeneration observed in Aβ42art flies was further confirmed by confocal analysis.
Islet conformation was studied by confocal analysis in consecutive slices stained for insulin and glucagon.
Next, we analyzed RCAN1 expression by confocal analysis and immunoblotting.
Consistently, the above results were also confirmed by confocal analysis.
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